Combining Headspace Solid-Phase Microextraction with Internal Benchmarking to Determine the Elimination Kinetics of Hydrophobic UVCBs

Substances classified as unknown or variable composition, complex reaction products biological origin (UVCB) present a challenge for environmental hazard and risk assessment. Here, we novel approach whole-substance bioconcentration testing applied to cedarwood oil—an essential oil composed of volatile, hydrophobic organic chemicals. The method yields whole-body elimination rate constants mixture constituents. Our combines in vivo dietary fish exposure with internal benchmarking headspace solid-phase microextraction (HS-SPME) equilibrium sampling followed by suspect-screening analysis. We quantified depuration 13 individual constituents based on relative peak areas using gas chromatography (GC) coupled Orbitrap-mass spectrometry (MS) GC triple-quadrupole (QqQ)-MS. For seven available analytical standards, compared the results obtained from solvent extraction, clean-up, targeted GC–MS HS-SPME allowed automated sample extraction analyte enrichment while minimizing evaporative losses volatile target analytes reducing matrix interferences low-volatility organics. analysis enabled quantification without significantly reduced variability differences delivered dose between samples.